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编号	518
名称	Cleavage Site Prediction (PeptideCutter)
封面
描述	预测蛋白质序列中潜在的蛋白酶或化学试剂切割位点。模块基于PeptideCutter工具对应的文献资料复现。
标签	Biologics Molecular Property Cleavage Enzyme Antibody Protein Peptide Developability
作者	Elisabeth Gasteiger
发布时间	2026-03-10 15:06:37
参考文献	Gasteiger, E. et al. (2005). Protein Identification and Analysis Tools on the ExPASy Server. In: Walker, J.M. (eds) The Proteomics Protocols Handbook. Springer Protocols Handbooks. Humana Press.

Enzyme / Chemical Name	Abbrev	P4	P3	P2	P1	P1’	P2’
Arg-C proteinase	ArgC	-	-	-	R	-	-
Asp-N endopeptidase	AspN	-	-	-	-	D	-
Asp-N endopeptidase + N-terminal Glu	AspN+AspGluN	-	-	-	-	D or E	-
BNPS-Skatole	BNPS	-	-	-	W	-	-
Caspase 1	Casp1	F,W,Y or L	-	H,A or T	D	not P,E,D,Q,K or R	-
Caspase 2	Casp2	D	V	A	D	not P,E,D,Q,K or R	-
Caspase 3	Casp3	D	M	Q	D	not P,E,D,Q,K or R	-
Caspase 4	Casp4	L	E	V	D	not P,E,D,Q,K or R	-
Caspase 5	Casp5	L or W	E	H	D	-	-
Caspase 6	Casp6	V	E	H or I	D	not P,E,D,Q,K or R	-
Caspase 7	Casp7	D	E	V	D	not P,E,D,Q,K or R	-
Caspase 8	Casp8	I or L	E	T	D	not P,E,D,Q,K or R	-
Caspase 9	Casp9	L	E	H	D	-	-
Caspase 10	Casp10	I	E	A	D	-	-
Chymotrypsin-high specificity (C-term to [FYW], not before P)	Ch_hi	-	-	-	F or Y	not P	-
		-	-	-	W	not P	-
Chymotrypsin-low specificity (C-term to [FYWML], not before P)	Ch_lo	-	-	-	F,L or Y	not P	-
		-	-	-	W	not M or P	-
		-	-	-	M	not P or Y	-
		-	-	-	H	not D,M,P or W	-
Clostripain (Clostridiopeptidase B)	Clost	-	-	-	R	-	-
CNBr	CNBr	-	-	-	M	-	-
Enterokinase	EK	D or E	D or E	D or E	K	-	-
Factor Xa	FXa	A,F,G,I,L,T,V or M	D or E	G	R	-	-
Formic acid	HCOOH	-	-	-	D	-	-
Glutamyl endopeptidase	GluC	-	-	-	E	-	-
GranzymeB	GzmB	I	E	P	D	-	-
Hydroxylamine (NH2OH)	Hydro	-	-	-	N	G	-
Iodosobenzoic acid	Iodo	-	-	-	W	-	-
LysC	LysC	-	-	-	K	-	-
LysN	LysN	-	-	-	-	K	-
Neutrophil elastase	Elast	-	-	-	A or V	-	-
NTCB (2-nitro-5-thiocyanobenzoic acid)	NTCB	-	-	-	-	C	-
Pepsin (pH1.3)	Pn1.3	-	not H,K or R	not P	not R	F or L	not P
		-	not H,K or R	not P	F or L	-	not P
Pepsin (pH>2)	Pn2p	-	not H,K or R	not P	not R	F,L,W or Y	not P
		-	not H,K or R	not P	F,L,W or Y	-	not P
Proline-endopeptidase[*]	Prol	-	-	H,K or R	P	not P	-
Proteinase K	ProtK	-	-	-	A,E,F,I,L,T,V,W or Y	-	-
Staphylococcal peptidase I	Staph	-	-	not E	E	-	-
Tobacco etch virus protease	TEV	-	Y	-	Q	G or S	-
Thermolysin	Therm	-	-	-	not D or E	A,F,I,L,M or V	not P
Thrombin	Throm	-	-	G	R	G	-
		A,F,G,I,L,T,V or M	A,F,G,I,L,T,V,W or R	P	R	not D or E	not D or E
Trypsin	Tryps	-	-	-	K or R	not P	-
		-	-	W	K	not P	-
		-	-	M	R	not P	-

Enzyme Name	P4	P3	P2	P1	P1’	P2’
Trypsin	-	-	C or D	K	D	-
Trypsin	-	-	C	K	H or Y	-
Trypsin	-	-	C	R	K	-
Trypsin	-	-	R	R	H or R	-

Chain ID	Name of enzyme	No. of cleavages	Positions of cleavage sites
seq_1	Arg-C proteinase	1	14
seq_1	Asp-N endopeptidase	1	2
seq_1	Asp-N endopeptidase + N-terminal Glu	2	2, 6
seq_1	BNPS-Skatole	1	4
seq_1	Chymotrypsin-high specificity (C-term to [FYW], not before P)	1	4
seq_1	Chymotrypsin-low specificity (C-term to [FYWML], not before P)	2	4, 6
seq_1	Clostripain (Clostridiopeptidase B)	1	14
seq_1	Formic acid	1	3
seq_1	Glutamyl endopeptidase	1	7
seq_1	Iodosobenzoic acid	1	4
seq_1	LysC	1	1
seq_1	Neutrophil elastase	1	13
seq_1	NTCB (2-nitro-5-thiocyanobenzoic acid)	1	4
seq_1	Pepsin (pH>2)	1	4
seq_1	Proteinase K	5	2, 4, 7, 8, 13
seq_1	Staphylococcal peptidase I	1	7
seq_1	Thermolysin	2	1, 12
seq_1	Trypsin	1	1
seq_2	Asp-N endopeptidase + N-terminal Glu	3	2, 11, 17
seq_2	BNPS-Skatole	2	2, 16
seq_2	Chymotrypsin-high specificity (C-term to [FYW], not before P)	5	2, 5, 11, 16, 17
seq_2	Chymotrypsin-low specificity (C-term to [FYWML], not before P)	9	1, 2, 5, 6, 9, 10, 11, 16, 17
seq_2	CNBr	1	6
seq_2	Glutamyl endopeptidase	3	3, 12, 18
seq_2	Iodosobenzoic acid	2	2, 16
seq_2	LysC	2	13, 15
seq_2	LysN	2	12, 14
seq_2	Neutrophil elastase	1	4
seq_2	NTCB (2-nitro-5-thiocyanobenzoic acid)	1	13
seq_2	Pepsin (pH1.3)	3	4, 5, 10
seq_2	Pepsin (pH>2)	4	4, 5, 10, 16
seq_2	Proteinase K	10	1, 2, 3, 4, 5, 11, 12, 16, 17, 18
seq_2	Staphylococcal peptidase I	3	3, 12, 18
seq_2	Thermolysin	3	4, 5, 10
seq_2	Trypsin	2	13, 15

字段	说明
Chain ID	序列名称。如果名称有重复时，会在原名称上添加上_dup1、如：A_dup1，`1`对应就是重复的次数。
Name of enzyme	蛋白酶/化学试剂名称，用于标识采用哪一种切割规则（例如 `Arg-C proteinase`、`Asp-N endopeptidase`、`BNPS-Skatole`、`CNBr` 等）。
No. of cleavages	该酶/试剂在对应序列上预测到的切割次数（切割位点数量）。应与 `Positions of cleavage sites` 中列出的位点个数一致。
Positions of cleavage sites	切割位点在序列中的位置编号列表。用逗号 + 空格分隔（例如 `2, 4, 7`）。酶或化学试剂的切割发生在序列对应位置之后。

Cleavage Site Prediction (PeptideCutter)

Cleavage Site Prediction (PeptideCutter)

参数说明

Input File

Enzymes

结果说明

参考文献