siRNA Designer

简介

siRNA Designer基于靶点基因序列，设计siRNA分子序列。该方法考虑了多条siRNA设计规则，如下：

• 36% < GCpercent < 52%
• no internal short repeats
• no GC stretches (more than 10 GC contigous repeats)
• 5’ end of the guide RNA is A/U
• 5’ end of the passenger RNA is G/C
• at least 4 A/U residues in the last 7bp of the 5’ end of the guide
• No G at position 13 of the passenger
• A/U at position 19 of the passenger
• G/C at position 19 in guide

参数说明

RNA FASTA File

靶点基因序列，支持多条，FASTA格式。

结果说明

输出结果文件为siRNAcandidates_序列名称.csv，包含信息如下：